IGEM:Stanford/2009/Notebook/Personal Notebook/2009/08/11

Suzie's iGEM Project

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Entries Daily Work Goals Revise diagram breakdowns for Jerome Revise vBOL symbols Either research Th-17 to NO pathway or work on wiki Project / Accomplishments Looks like transformations worked!!!! Yay! (1.1b and 2.1a) Mini-preps of SoxR/S cultures and pcr on 1.1b and 2.1a Need to sequence 1.2a Made revised constructions for Device 1 Device1 Diagram- Revision1 New schematics for Diagrams (see right) Overall Device Schematic right Device 1 Schematic vBOL symbols revised PDF of new vBOL symbols Problems Simple schematic for Device 2.... Alternative Solutions Events Meeting w/ Jerome for the presentation Show Naive CD4+ cells leading to T-cells? Testing line w/ filtering T-cells Numbers Rough percentage of amounts of T-cells (normal, non-recognizing, self-recognizing...) Why don't some self-recognizing t-cells undergo apoptosis? Circuit diagram Make text bigger, animation,... Take out IDO and dendritic cells? Shorter descriptions More visual support and animation Specifically on slides describing why we need device 2 Device 2 parts breakdown- need separate promoter Visual- pyramid w/ age group and Immunology section- still too complicated More pictures Start w/ what the immune system "is" as a definition? Very general Stay away from capital letters Differentiate acquired and innate immune responses- mention vaccines! Talk more about acquired w/ animation (fun can be good) What is an antigen? What is an antibody? Scheme is nice- could be more simple Take away Tc cells Selective filtering- make a factory line and assembly line Define autoimmune diseases w/ respect to Self-recognizing t-cells Start w/ epidemiology then immune response, then back to IBD w/ respect to the immune system Mention system therapy vs. cure Showing the cause of inflammation itself.... b-bells, t-cells, and plasma cells (igE?)- Show transportation- lymph, vs blood, vs epithelial crossing Where are Naïve CD4+ cells? Needs to be in the place of activity!!! email Ariana or Professor Steinman Meeting w/ Christine for Stanford News at 2pm Interviews, vidoes, and revised article Looks good! FAB at 4:00 Trp looks better, but not perfect yet... More modeling help needed? IBD Overview Be upfront of assumptions and parameters We assume it is t-cell ratios for inflammation Questions to answer Where are Naive CD4+ cells? What is the "cause" of IBD? Why don't medicines target t-cells? Also, Future- how can we biosynthesize 5MT? Comments Questions to be answered…. Genetic component of the disease? Mouse model of IBD to test linear relationship of Th-17 and Treg cells Suicide device? Do people take RA for treatment? The acid form of Vitamin A, all-trans retinoic acid or ATRA, has been made into Tretinoin, which Do people take Il-6 for treatment? Other uses? Where are Naïve CD4+ cells? Needs to be in the place of activity!!! vBOL, are there names for the individual pictures Weekly Goals