IGEM:Stanford/2009/Groups/Team CAM/microbots

• Benefits of Bactofection by Microbots
  • Krusch J Gene 2003
• Riboswitches [Anusuya Ramasubramanian]
  • Why Riboswitches?
  • Characterize input/output signal
  • What riboswitch would you use?
  • What signaling molecule would you use
• Surface Protein Expression (B+J)
  • What other bacterial surface receptors exist?
    • Look into InlB Hamon Nat Rev Micro 2006
  • What antigens are unique to cancer cells?
• Nanoparticle production (B+J)
  • Read supplement of Akin Nat Nano 2007
  • Costs? Difficulty?
• Model Organisms [Mark Fang]
  • What human cell lines do we need?
  • What vector would we use
    • Why?
    • L. monocytogenes?
      • Krusch J Gene 2003
      • Pilgrim Gene Ther 2002
      • Safety? Angelakopoulos Infect Immun 2002
    • Ecoli?
      • Bactofection by E.coli
      • Larsen Gene Ther 2008
• Previous iGEM Projects
  • Bacteria targeting Harvard 07
  • Safe Bacterium Bactoblood 07
  • Killing phage Heidelberg 08
  • Riboswitches Melbourne 08
  • Making new receptors Sheffield 08
  • Gene delivery to yeast U Washinton 08
  • http://openwetware.org/images/0/00/Visualization_of_tumors_and_metastases_in_live_animals_with_bacteria_and_vaccinia_virus.pdf