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September 5, 2007

  • Made more 1X TAE buffer
    • Added 40mL of 50X TAE buffer to 4 L flask
    • Added 1960mL dH20 and mixed
  • Restriction digest on crp* and P1010 in psB1A2
    • cut with EcoR1 and SpeI
    • Started incubating at 6:45pm
  • Ran gel on restriction products and gel extracted psB1A2