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September 3, 2007

  • Ran gel on PCR products
  • PCR purified the 2 PCR rxns which worked:
    • 0.01uL crp* template Pfu Polymerase
    • 1.0uL crp* template Phusion Polymerase
  • Restricted the 2 PCR purified samples along with P1010 in psB1A2
    • Cut with E+S, 20uL digests
  • Ran gel on restrictions to extract psB1A2 and confirm that I still have crp*
  • Ran out of TAE buffer and Tien's lab is closed and locked. Will get more TAE and re-restrict tomorrow or wednesday.