IGEM:PennState/Labbook/NoahJohnson/2007-9-20

September 20, 2007

• DH5a competent cell prep

• Ligation labelling system:
  • 1: Tube 1 crp* & Tube 1 psB1A2 (Fridge ligation)
  • 2: Tube 2 crp* & Tube 4 psB1A2 (Fridge ligation)
  • 3: (-) Tube 1 psB1A2 (Fridge ligation)
  • 4: Tube 3 crp* & Tube 1 psB1A2 (Benchtop ligation)
  • 5: Tube 4 crp* & Tube 4 psB1A2 (Benchtop ligation)
  • 6: (-) Tube 4 psB1A2 (Benchtop ligation)

• Transformation:
  • 3uL ligation mixture in 50uL DH5a competent cells
  • Will do 2 transformations for each ligation, one with todays competent cells (New) and one with yesterday's (Old).
  • Plated on LB Amp

• Made 500mM filter-sterilized glucose soln for SOC:
  • 0.9g Glucose (MW=180.16 g/mol) added to 10mL dH20
  • Filter sterilized and put in 15mL Falcon tube
  • Can be found in rack in fume hood

6-11:30