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August 20, 2007

PCR purified the working crp* rxn from friday (0.1uL template crp* DNA with Phusion polymerase)

Restriction digest of crp* and P1010 in psB1A2 with EcoR1 and SpeI

Ran gel of restrictions:

  • All 4 crp* digests worked
  • none of the psB1A2 digests seemed to work