IGEM:PennState/Labbook/NoahJohnson/2007-7-4

July 4, 2007

• Poured gel
• Ligation (Started at 12:50):
  • (+) 20uL 1741015-PSB1A2
    • 2uL T4 Ligase buffer
    • 4ul Vector (P1010 in PSB1A2+S+E)
    • 12uL Insert (I741015+S+E)
    • 1uL T4 DNA Ligase
    • 1uL H2O
  • (-) 20uL PSB1A2
    • 2uL T4 Ligase buffer
    • 4uL Vector
    • 1uL T4 DNA Ligase
    • 13uL H2O

• Ran Transformation
• Plated on LB Amp

9:30-3:30