IGEM:PennState/Labbook/NoahJohnson/2007-6-22

June 22, 2007

• Ran Gel (start 11:35am)
  • Well 3- DNA Ladder
  • Well 4- B0015 Lig. (-)
  • Well 5- B0015 + E0040 Lig.
  • Well 6- B0015 Vect. GE
  • Well 7- E0040 Ins. GE
  • Well 8- DNA Ladder

• Performed Transformation
  • puC19 into competent cells
  • Three trials using:
    • New DH5α competent cells
    • Old DH5α competent cells
    • New DB3.1 competent cells

9:30-5