IGEM:PennState/2007/Progress Cloning

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Primers:

Promoter Region Primers

Double Promoter

Full Promoter AtoF F-GGAATTCGCGGCCGCTTCTAGAGTATTGAACTCCATAATCAGGTAATG

  • TmC 61

R-CTGCAGCGGCCGCTACTAGTATGGTGTAGGGCCTTCTGTAG

  • TmC 62

Full Promoter FtoA F-GGAATTCGCGGCCGCTTCTAGAGTGGTGTAGGGCCTTCTGTA

  • TmC 61

R-CTGCAGCGGCCGCTACTAGTATATTGAACTCCATAATCAGGTAATG

  • TmC 58

ORF Promoter AtoF

    • MISSING**

ORF Promoter FtoA F-GGAATTCGCGGCCGCTTCTAGAGAACCAATTTTTAGCAACTAAACAG

  • TmC 58

R-CTGCAGCGGCCGCTACTAGTAATGGATGATGTCGTAATATTGG

  • TmC 59

Single Promoter

CRP XylR XylR Fis F- GGAATTCGCGGCCGCTTCTAGAGTCCCTTTCAGTTGCTCAAT

  • TmC 58

R- CTGCAGCGGCCGCTACTAGTAGGTGTAGGGCCTTCTGTAG

  • TmC 60

XYLR XYLR FIS F-GGAATTCGCGGCCGCTTCTAGAGTTACGTTTATCGCGGTGATT

  • TmC 59

R-CTGCAGCGGCCGCTACTAGTAGGTGTAGGGCCTTCTGTAG

  • TmC 60

CRP

F-GGAATTCGCGGCCGCTTCTAGAGATGGTGCTTGGCAAACC

  • TmC 60

R-CTGCAGCGGCCGCTACTAGTATTAACGAGTGCCGTAAAC

  • TmC 57

XylR

XylR with Promoter RBS and terminator: Right F-GGAATTCGCGGCCGCTTCTAGAGGCAACCAAACGCCGTTCTTG

  • TmC 65

R-CTGCAGCGGCCGCTACTAGTAATTGCATTTCCTTGAGCCTTATCC

  • TmC 63

XylR with Promoter RBS and terminator: Left F-GGAATTCGCGGCCGCTTCTAGAGATTGCATTTCCTTGAGCCTTATCC

  • TmC 63

R-CTGCAGCGGCCGCTACTAGTAGCAACCAAACGCCGTTCTTG

  • TmC 65

XylR ORF Right

    • Missing**

XylR ORF Left

    • Missing**
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