IGEM:PennState/2007/Labbook/GarrettTobin/2007-8-2
August 2nd
- Miniprep B0034 (2)
- Digest those with 2 xylR minipreps from yesterday
- Ran Gel
- Designed Primers for xylR Total and middle for sequencing
xylR total 3732929..3734180
Extended for primers 3732860…3734210
TCGTTAAAGG TGCGATTCTG TTGCTGGCAG TATGGATGGA CTCCGCAACC AAACGCCGTT
CTTGATTTTG ATAAAAATTT TCTCAAAGCC GGTTACGTAT TACCGGTTTT GAGTTTTTGC
ATGATTCAGC AGGAAAAGAA CCatgTTTAC TAAACGTCAC CGCATCACAT TACTGTTCAA
TGCCAATAAA GCCTATGACC GGCAGGTAGT AGAAGGCGTA GGGGAATATT TACAGGCGTC
ACAATCGGAA TGGGATATTT TCATTGAAGA AGATTTCCGC GCCCGCATTG ATAAAATCAA
GGACTGGTTA GGAGATGGCG TCATTGCCGA CTTCGACGAC AAACAGATCG AGCAAGCGCT
GGCTGATGTC GACGTCCCCA TTGTTGGGGT TGGCGGCTCG TATCACCTTG CAGAAAGTTA
CCCACCCGTT CATTACATTG CCACCGATAA CTATGCGCTG GTTGAAAGCG CATTTTTGCA
TTTAAAAGAG AAAGGCGTTA ACCGCTTTGC TTTTTATGGT CTTCCGGAAT CAAGCGGCAA
ACGTTGGGCC ACTGAGCGCG AATATGCATT TCGTCAGCTT GTCGCCGAAG AAAAGTATCG
CGGAGTGGTT TATCAGGGGT TAGAAACCGC GCCAGAGAAC TGGCAACACG CGCAAAATCG
GCTGGCAGAC TGGCTACAAA CGCTACCACC GCAAACCGGG ATTATTGCCG TTACTGACGC
CCGAGCGCGG CATATTCTGC AAGTATGTGA ACATCTACAT ATTCCCGTAC CGGAAAAATT
ATGCGTGATT GGCATCGATA ACGAAGAACT GACCCGCTAT CTGTCGCGTG TCGCCCTTTC
TTCGGTCGCT CAGGGCGCGC GGCAAATGGG CTATCAGGCG GCAAAACTGT TGCATCGATT
ATTAGATAAA GAAGAAATGC CGCTACAGCG AATTTTGGTC CCACCAGTTC GCGTCATTGA
ACGGCGCTCA ACAGATTATC GCTCGCTGAC CGATCCCGCC GTTATTCAGG CCATGCATTA
CATTCGTAAT CACGCCTGTA AAGGGATTAA AGTGGATCAG GTACTGGATG CGGTCGGGAT
CTCGCGCTCC AATCTTGAGA AGCGTTTTAA AGAAGAGGTG GGTGAAACCA TCCATGCCAT
GATTCATGCC GAGAAGCTGG AGAAAGCGCG CAGTCTGCTG ATTTCAACCA CCTTGTCGAT
CAATGAGATA TCGCAAATGT GCGGTTATCC ATCGCTGCAA TATTTCTACT CTGTTTTTAA
AAAAGCATAT GACACGACGC CAAAAGAGTA TCGCGATGTA AATAGCGAGG TCATGTTGTA
GCGATGATGA GAATTGTCGG CGTCACATCA G
Primers
TOTAL:
Front Gaattcgcggccgcttctagag TCGTTAAAGG TGCGATTCTG
End
AATTGTCGG CGTCACATCAG tactagtagcggccgctgcag
Reverse complement Ctgcagcggccgctactagta CTGATGTGACGCCGACAATT
Middle for sequencing: Forward GCTACAAACG CTACCACCGC
Reverse Compliment GCGGTGGTAGCGTTTGTAGC
