IGEM:Peking University/2008/Notebook/Group 2/2008/06/27

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0627

TITLE: PCR-pPT-Gal4-Transtart 20ul*6
Volume (μL)
Template 0.5
dNTP 2
Enzyme 0.5
Buffer 2
F-Primer 0.2
R-Primer 0.2
ddH2O 14
conditions
Temperature Time Length(0:00:00)
94°C 02:00
94°C 01:00 4 cycles
50°C 02:00 4 cycles
72°C 05:00 4 cycles
94°C 01:00 30 cycles
55.2,55.6,57.6,59.5,61.3,63.9°C 01:00 30 cycles
72°C 03:00 30 cycles
72°C 10:00
4°C Hold

Enzyme cutting-T-Gal4-XbaI-20*6 μL
Volumn(μL)
DNA 2
XbaI 1
M Buffer 2
BSA 2
ddH2O 13

Enzyme cutting-T-Gal4-SpeI-HindIII-20*4 μL
Volumn(μL)
DNA 2
SpeI 1
HindIII 1
M Buffer 2
ddH2O 14