Primer design
- Primer 1
- Primer name: F-Gal4-HindIII-Apal
- Sequence(5'to3'):AAAAAGCTTGGGCCCTTTTAAGAGAGGACAGAGAAGCAAGCCTC
- Tm:64.1°C
- Primer 2
- Primer name: R-Gal4-EcoRI-Xhol
- Sequence(5'to3'):GGGGAATTCCTCGAGTGCGGGGTTTTTCAGTATCTACGATTCATT
- Tm:64.0°C
PCR
TITLE: PCR-Budding Yeast Genome-Gal4-K.O.D 50ul
|
Volume (μL)
|
Template (Budding Yeast Genome) |
1
|
dNTP |
5
|
Enzyme |
1
|
Buffer |
5
|
MgSO4(For KOD) |
2
|
F-Gal4-HindIII-ApaI |
1
|
R-Gal4-EcoRI-Xhol |
1
|
ddH2O |
34
|
- conditions
Temperature(&&&) |
Time Length(0:00:00)
|
94°C |
07:00
|
94°C |
00:30 |
30 cycles
|
59°C |
00:30 |
30 cycles
|
55°C |
00:30 |
30 cycles
|
72°C |
03:00 |
30 cycles
|
72°C |
07:00
|
4°C |
Hold
|
|