IGEM:Peking University/2008/Notebook/Group 1/2008/07/19

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PKU_080719_G1_1


Transformation-single/double ligation-pGREG504-pAID-lac2/Control(C1~C5)



Gel running-enzyme cutting product-pADH-lac1/lacS/tet(single/double ligation)
File:PKU-080719-digestion for single and double ligation-1st.tif



Gel extraction-enzyme cutting product-pADH-lac1/lacS/tet(single/double ligation)
Changing kit (QIAquick)
Gel running-check extraction product
File:PKU-080719-check-digestion single or double ligation-1ST-AID.tif
lanes:1BS|SBS|TBS|AII|PGSX|1SX|SSX|TSX



Single Ligation-pGREG504-pAID-lac1/S/tet
Volume (μL)
promoter 4
pGREG504 1
ligation buffer 5


Double Ligation-pGREG504-pAID-lac1/S/tet-AID
Volume (μL)
promoter 3
AID 3
pGREG504 1
ligation buffer 7


Control C2-1
Volume (μL)
promoter(single ligation) 4
ddH2O 1
ligation buffer 5


Control C2-2
Volume (μL)
promoter(double ligation) 3
ddH2O 4
ligation buffer 7


Control C2-3
Volume (μL)
promoter(double ligation) 4
AID 3
ddH2O 1
ligation buffer 7


Control C2-4
Volume (μL)
ddH2O 5
ligation buffer 5