PKU_080719_G1_1
- Transformation-single/double ligation-pGREG504-pAID-lac2/Control(C1~C5)
- Gel running-enzyme cutting product-pADH-lac1/lacS/tet(single/double ligation)
- Gel extraction-enzyme cutting product-pADH-lac1/lacS/tet(single/double ligation)
- Changing kit (QIAquick)
- Gel running-check extraction product
- lanes:1BS|SBS|TBS|AII|PGSX|1SX|SSX|TSX
- Single Ligation-pGREG504-pAID-lac1/S/tet
|
Volume (μL)
|
promoter |
4
|
pGREG504 |
1
|
ligation buffer |
5
|
- Double Ligation-pGREG504-pAID-lac1/S/tet-AID
|
Volume (μL)
|
promoter |
3
|
AID |
3
|
pGREG504 |
1
|
ligation buffer |
7
|
- Control C2-1
|
Volume (μL)
|
promoter(single ligation) |
4
|
ddH2O |
1
|
ligation buffer |
5
|
- Control C2-2
|
Volume (μL)
|
promoter(double ligation) |
3
|
ddH2O |
4
|
ligation buffer |
7
|
- Control C2-3
|
Volume (μL)
|
promoter(double ligation) |
4
|
AID |
3
|
ddH2O |
1
|
ligation buffer |
7
|
- Control C2-4
|
Volume (μL)
|
ddH2O |
5
|
ligation buffer |
5
|
|