IGEM:Peking University/2008/Notebook/Group 1/2008/07/08

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PKU_080708_G1_1



Cultivation- E.Coli(liguid-Amp+)

Plasmid Miniprep-pADH-lac1-T

Enzyme cutting-pADH-lac1-T
Volume (μL)
pADH-lac1-T 15
SacI 5 *2
XhoI 5
M Buffer 10
ddH2O 65


Volume (μL)
pADH-lac1-T 15
SacI 2 *2
XhoI 2
HindIII 2
M Buffer 10
ddH2O 69



Enzyme cutting-pADH-lac?-AIDwrong
Volume (μL)
pADH-lac?-AIDwrong 10
BamHI 2 *3
XhoI 2
K Buffer 10
ddH2O 76



Enzyme cutting-pGREG504
Volume (μL)
pGREG504 10
SacI 5
XhoI 5
M Buffer 10
ddH2O 70



Gel running