IGEM:Peking University/2008/Notebook/Group 1/2008/05/27

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PKU_080527_G1_1


PCR- pGREG504/(pESC-hAID)-hAID-50ul
Volume (μL)
pGREG504/(pESC-hAID) 1
transtart taq 1
transtart buffer 5
dNTP 3
F-Primer 1
R-Primer 1
ddH2O 58


conditions
Temperature(°C) Time Length(0:00:00)
94 04:00
50 02:00
72 02:00
94 01:00 30 cycles
56 02:00 30 cycles
72 01:00 30 cycles
72 05:00

Enzyme cutting-pGREG506'/(pADH-lacO1)-ClaI-BamHI
Volume (μL)
pGREG506'/(pADH-lacO1) 5
ClaI 0.2
BamHI 0.2
K Buffer 2
ddH2O 12.6

didn't work