IGEM:Peking University/2008/Experiment/PKU 080708 G1 1
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PKU_080708_G1_1
- Cultivation- E.Coli(liguid-Amp+)
- Plasmid Miniprep-pADH-lac1-T
- Enzyme cutting-pADH-lac1-T
| Volume (μL) | ||
| pADH-lac1-T | 15 | |
| SacI | 5 | *2 |
| XhoI | 5 | |
| M Buffer | 10 | |
| ddH2O | 65 |
| Volume (μL) | ||
| pADH-lac1-T | 15 | |
| SacI | 2 | *2 |
| XhoI | 2 | |
| HindIII | 2 | |
| M Buffer | 10 | |
| ddH2O | 69 |
- Enzyme cutting-pADH-lac?-AIDwrong
| Volume (μL) | ||
| pADH-lac?-AIDwrong | 10 | |
| BamHI | 2 | *3 |
| XhoI | 2 | |
| K Buffer | 10 | |
| ddH2O | 76 |
- Enzyme cutting-pGREG504
| Volume (μL) | |
| pGREG504 | 10 |
| SacI | 5 |
| XhoI | 5 |
| M Buffer | 10 |
| ddH2O | 70 |
- Gel running
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