IGEM:Peking University/2008/Experiment/PKU 0807022 G1 1
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PKU_080722_G1_1
- Positive transformation-pGREG504(new)/DH5α/JM109
- Picking up clonies
- Gel running and testing-hypothesis-promoter's ligation-(lac1/lacS/tet)(SB/SX)AID(BX)
- result:no band and snear
- Sequencing-ligation product(yesterday)
- Ligation test-pGREG504-promoter-old/new ligation buffer
- Enzyme cutting and testing-pGREG504-single(sacI/XhoI)-double(sacI&XhoI)
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