IGEM:Peking University/2008/Experiment/PKU 0807019 G1 1
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PKU_080719_G1_1
- Transformation-single/double ligation-pGREG504-pAID-lac2/Control(C1~C5)
- Gel running-enzyme cutting product-pADH-lac1/lacS/tet(single/double ligation)
- Gel extraction-enzyme cutting product-pADH-lac1/lacS/tet(single/double ligation)
- Changing kit (QIAquick)
- Gel running-check extraction product
- lanes:1BS|SBS|TBS|AII|PGSX|1SX|SSX|TSX
- Single Ligation-pGREG504-pAID-lac1/S/tet
| Volume (μL) | |
| promoter | 4 |
| pGREG504 | 1 |
| ligation buffer | 5 |
- Double Ligation-pGREG504-pAID-lac1/S/tet-AID
| Volume (μL) | |
| promoter | 3 |
| AID | 3 |
| pGREG504 | 1 |
| ligation buffer | 7 |
- Control C2-1
| Volume (μL) | |
| promoter(single ligation) | 4 |
| ddH2O | 1 |
| ligation buffer | 5 |
- Control C2-2
| Volume (μL) | |
| promoter(double ligation) | 3 |
| ddH2O | 4 |
| ligation buffer | 7 |
- Control C2-3
| Volume (μL) | |
| promoter(double ligation) | 4 |
| AID | 3 |
| ddH2O | 1 |
| ligation buffer | 7 |
- Control C2-4
| Volume (μL) | |
| ddH2O | 5 |
| ligation buffer | 5 |
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