IGEM:Peking University/2008/Experiment/PKU 0807017 G1 1
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PKU_080717_G1_1
- PCR- pGREG504-pADH-(lac1/lac2/lacS/tet)-50ul
- Using program lac12MQT
- Enzyme cutting-pADH-(lac1/lac2/lacS/tet)(PCR product)
| Volume (μL) | |
| Promoter | 15 |
| BamHI | 2 |
| SacI | 2 |
| 0.5×K Buffer | 5 |
| ddH2O | 76 |
| Volume (μL) | |
| Promoter | 15 |
| SacI | 5 |
| XhoI | 5 |
| 1×M Buffer | 10 |
| ddH2O | 65 |
- Enzyme cutting-AID
| Volume (μL) | |
| AID | 15 |
| BamHI | 2 |
| XhoI | 2 |
| 1×K Buffer | 10 |
| ddH2O | 71 |
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