IGEM:Peking University/2008/Experiment/PKU 0807014 G1 1
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PKU_080714_G1_1
- Plasmid Miniprep-pGREG504-pADH-(lac1-1/lac1-2/lac2-1/lac2-2/Control1/Control2/Control3)
- Enzyme cutting-pADH-(lacS/tet)(PCR product)
| Volume (μL) | |
| pADH-(lacS/tet) | 14 |
| SacI | 2 |
| XhoI | 2 |
| M Buffer | 10 |
| ddH2O | 72 |
- Enzyme cutting-pGREG504-1
| Volume (μL) | |
| pGREG504-1 | 4 |
| SacI | 2 |
| XhoI | 2 |
| M Buffer | 10 |
| ddH2O | 82 |
- lanes:lacS|tet|vector|marker1|marker2
- Enzyme cutting-pGREG504-pADH-(lac1-1/lac1-2/lac2-1/lac2-2/Control1/Control2/Control3)
| Volume (μL) | |
| pGREG504-pADH-(lac1-1/lac1-2/lac2-1/lac2-2/Control1/Control2/Control3) | 5 |
| SacI | 1 |
| XhoI | 1 |
| M Buffer | 2 |
| ddH2O | 11 |
| Volume (μL) | |
| pGREG504-pADH-(lac1-1/lac1-2/lac2-1/lac2-2) | 5 |
| SphI | 0.2 |
| XhoI | 0.2 |
| H Buffer | 2 |
| ddH2O | 12.6 |
| Volume (μL) | |
| pGREG504-pADH-(Control1/Control2/Control3) | 5 |
| BamHI | 0.2 |
| XhoI | 0.2 |
| K Buffer | 2 |
| ddH2O | 12.6 |
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