IGEM:Peking University/2008/Experiment/PKU 080527 G1 1
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PKU_080527_G1_1
- PCR- pGREG504/(pESC-hAID)-hAID-50ul
| Volume (μL) | |
| pGREG504/(pESC-hAID) | 1 |
| transtart taq | 1 |
| transtart buffer | 5 |
| dNTP | 3 |
| F-Primer | 1 |
| R-Primer | 1 |
| ddH2O | 58 |
- conditions
| Temperature(°C) | Time Length(0:00:00) | |
| 94 | 04:00 | |
| 50 | 02:00 | |
| 72 | 02:00 | |
| 94 | 01:00 | 30 cycles |
| 56 | 02:00 | 30 cycles |
| 72 | 01:00 | 30 cycles |
| 72 | 05:00 |
- Enzyme cutting-pGREG506'/(pADH-lacO1)-ClaI-BamHI
| Volume (μL) | |
| pGREG506'/(pADH-lacO1) | 5 |
| ClaI | 0.2 |
| BamHI | 0.2 |
| K Buffer | 2 |
| ddH2O | 12.6 |
didn't work
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