IGEM:Peking University/2008/Experiment/PKU 080519 G1 1

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PKU_080519_G1_1

Cultivation- E.Coli(liguid-Amp+)
Plasmid Miniprep-pGREG504/pADH
Enzyme cutting-pGREG504/pADH-EcoRV
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Volume (μL)
pGREG504/pADH 5
EcoRV 0.2 *13
H Buffer 2
ddH2O 12.8


Enzyme cutting-pGREG504/pADH-SacI-BamHI
Volume (μL)
pGREG504/pADH 5
SacI 0.2 *13
BamHI 0.2
K Buffer 2
ddH2O 13.6


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