IGEM:Peking University/2008/Experiment/PKU 080513 G1 1

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PKU_080513_G1_1


PCR- pGREG505/(TEF-lacI-ADHlacS-GFP)-adhlac-50ul
File:PKU 080513 ADH-PCR-check.tif
Volume (μL)
pGREG505/(TEF-lacI-ADHlacS-GFP) 1
dNTP 5
transtart taq 1
transtart Buffer 5
F-Primer 1
R-Primer 1
ddH2O 36


conditions
Temperature(°C) Time Length(0:00:00)
94 05:00
94 00:30 30 cycles
50,55,60 00:30 30 cycles
72 01:30 30 cycles
72 07:00
Alarm 5 times
Hold 4°C




Enzyme cutting-DNA (pGREG503/504)-SacI-XhoI-20 μL
File:PKU 080513 pGREG504-cut.tif
Volumn(μL)
pGREG503/504 5
SacI 0.2
XhoI 0.2 *5
M Buffer 2
ddH2O 12.6
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