IGEM:Peking University/2008/Experiment/PKU 0716 G3 1
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PKU_080716_G3_1
- Colony PCR-AH109+pGREG503-LacI
- PCR system 15μL
| Volume (μL) | |
| buffer | 5 |
| Yeast | a few |
| dNTP | 1.5 |
| F-Primer | 0.3 |
| R-Primer | 0.3 |
| ddH2O | 11.4 |
- conditions
| Temperature(°C) | Time Length(0:00:00) | |
| 98 | 05:00 | |
| 94 | 00:30 | 35 cycles |
| 60 | 00:30 | 35 cycles |
| 72 | 02:30 | 35 cycles |
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