IGEM:Peking University/2008/Experiment/PKU 0605 G2 1
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- Enzyme cutting-pUC18-HindIII-EcoRI-20 μL
| Volumn(μL) | |
| pUC18 | 5 |
| HindIII | 0.2 |
| EcoRI | 0.2 |
| M Buffer | 2 |
| ddH2O | 12.6 |
- Enzyme cutting-Gal4-HindIII-EcoRI-50 μL
| Volumn(μL) | |
| Gal4 | 15 |
| HindIII | 0.2 |
| EcoRI | 0.2 |
| M Buffer | 5 |
| ddH2O | 29 |
- Ligation-pUC18/Gal4
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