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Rescue I7100 by gel

Ligation of I7100 with 434OR21/321/EGFP

digestion test of T1TE-T1T2-pcc010

This time I use XhoI and KpnI in M buffer

COlony PCR of I7100-434OR, EGFP

buffer 1.5uL

dNTP 1.5uL

primer 0.5uL each

easy Taq 0.1uL

dH2O 11uL

a total 15uL system


Big day! sequencing result back and we got all fragments right except CD3!