From OpenWetWare
Jump to: navigation, search

Precipitation of EGFP, 434OR21/321

rescue them afterwards and run a gel to check

PCR double repressors

This is a second time, just in case we used up all the products last time

Miniprep of T1TE-T1T2-pcc010

digestion test using ClaI and ScaI in H buffer, but failed

I am sure the plasmid is right, next I will change some other endonucleae to see!

PCR of parts for switch the third time

This time we have rescued most of the parts we need in the future!

Together with the first 2 times, we got every parts for at least once.

ligation of I7100 with SDY-EGFP, 434OR321/21

Transformation afterwards and overnight culivation