IGEM:Peking/2007/Switch-Notebook/2007-9-12
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Problem and Warning
Our Kana and Amp plates seems all wrong!!!
I have discarded them!
Ligation
pSB3K3 with so many fragments
Transformation
The previous ligation system.
Also some self-ligation control and some positive transformation aiming at resources of vectors
Re-digestion of all the fragments
We got these fragments from PCR yesterday