IGEM:Peking/2007/Switch-Notebook/2007-9-10

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Colony PCR

Finally we have some colonies that may turn out right

Rescue by precipitation of all fragments

Ex-Taq PCR of RS1 and EGFP

We got the two fragments

ligation

I7100 with CIind-

I13521 with OR21/321/EGFP

Forget self-ligation!