IGEM:Peking/2007/Switch-Notebook/2007-8-7

Ligation and transformation

T1TE^^(A/X)         lacZa-pLX003^^
lacZ-pLX003^^(A/X)  (self-ligation-control)

Digestion of plasmids

  2uL    T1T2-pCC002
  2uL    ClaI
  2uL    SalI
 10uL    10*H buffer
 84uL    H2O
----------------------
100uL    total

  2uL    SDY-EGFP=pLX007
  2uL    AatII
  2uL    XhoI
 10uL    T
 10uL    BSA
 74uL    H2O
----------------------
100uL    total