IGEM:Peking/2007/Switch-Notebook/2007-8-31
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Colony PCR of T1TE-T1T2-pcc010
primer: pcctestR and pkan-Kan-F
some colonies may be right!
Colony PCR of 434OR/21/321
primer: MBF and MBR
some colonies may be right, send to sequence.
Colony PCR of CD-plx012
some colonies may be right!
rescue by gel of I13521
I7100 miniprep after picking some colonies
digestion test afterwards using EcoRI/PstI in H buffer, overnight
