IGEM:Peking/2007/Switch-Notebook/2007-8-28
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Colony PCR of CD2, SulA, RS2, RD3 ligated with plx012
buffer 1.5uL
dNTP 1.5uL
dH2O 11uL
test-F/GFP-test-R 0.5uL each
easy taq 0.1uL
elongation time span of 1 minute
T1T2-pcc010 rescue by precipitation
T1TE-pMD18T rescue by gel eletrophoresis
transformation
CD2, sulA, RS2, RD3-plx012
positive transformation: CD1-plx012 and plx012 itself
plx012 and CD1-plx012 culivated in advance
miniprep of plx012 and CD1-plx012
double digestion test to prove its accuracy.
