IGEM:Peking/2007/Switch-Notebook/2007-8-20

digestion some plasmids

colony PCR of RD2, RD3, RS2, CD1

no one is right

ligation of lacI and pcc009

to prepare enough supplies

re-PCR some double promoers

CD1, SS1, RS1, RS2, RD2, RD3(68), RD3(70)

after rescue from the gel, run a electrophoresis to check:

CD1, SS1, RS1, RS2, RD2, RD3

re-digestion of GFP-plx007 3uL

usage: XhoI and XbaI in 100uL solution