IGEM:Peking/2007/Switch-Notebook/2007-8-19
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experimenter: WMC, CDZ
precipitation of RD3, RD2, RS2, CD1
but the electrophoresis shows no visible signs, which means failure
ligation
with GFP-plx007, all use the proportion 4:1
miniprep of SS3, RS3，all together 30
re-digestion of RD3, RD2, RS3, CD1
template: 20uL XhoI 2uL SpeI 2uL H beffer 10uL ddH2O 66uL for a shortage of template, we just use 10-15uL template in each solution
