IGEM:Peking/2007/Switch-Notebook/2007-8-10
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rescue by precipitation
9 fragments: lac, rec, sul, SS1-3, SD1-3, digested by XhoI/SpeI
1 vector: GFP-plx007, digested by XhoI/XbaI
miniprep
T1TE-plx007
T1TE-T1T2-pcc010
double digestion test applied to these two plasmids
sequencing results
change the primers to ensure the right result!
transformation
Prec, Plac, Psul, SS1-3,SD1-3 ligated with GFP-plx007 for 5 hours
SDY-EGFP-plx007 positive transformation
re-colony PCR of T1TE-T1T2-pcc010
forget to make the positive control! failed.
