IGEM:Peking/2007/Switch-Notebook/2007-7-31

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re-digestion of plx007/008/009

Only Plx009 seems to be right!

double digestion of plx009-lacZa

plx009 4uL

buffer H 6uL

XhoI/SalI 2uL each

H2O 46uL

Total 60uL system

Colony PCR of T1TE-pcc010

electrophoresis of plx009-lacZa

OK! plx009-lacZa is done!!!

plx008-6 and plx009-5 send to sequencing

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