IGEM:Peking/2007/Switch-Notebook/2007-7-25

From OpenWetWare
Jump to navigationJump to search

Experimenter: LD & CXY

Contents

Transformation

  • lacZa-pLX007/8/9
  • lacZa-pLX007/8/9 (self connection control)
  • RS1/2, RD1/2-pMD18-T
  • 10 in total!
  • Time: 10am

Colony PCR test of RS1/2, RD1/2-T bacteria

  • Time: 12pm

For each of RS1/2, RD1/2-T, 17 colonies are tested.

Select for incubation:

  • RD1: 1,4,6,11,17
  • RD2: 1,4,5,8,10
  • RS1: 3,4,6,8,11
  • RS2: 2,8,9,10,14