IGEM:Peking/2007/Switch-Notebook/2007-7-20
From OpenWetWare
Jump to navigationJump to search
Miniprep of pLx007-lacZa(8)
double digestion test of lacZa-plx007/008/009
method I
H buffer 2uL
XhoI/SalI 0.5uL each
plasmid 12uL
dH2O 5uL
total 20uL
Failed!
Method II
H buffer 2uL
ScaI/SalI 0.5uL each
plasmid 8uL
dH2O 9uL
total 20uL
for some reason in eletrophoresis, the result is not very good.
PCR Prec7-10 and Prec7-8
using our own plasmid as the template
ddH2O 36.75uL
buffer 5uL
dNTP 5uL
Ex-Taq 0.25uL
template 1uL
Rec-F/R 1uL each
Total 50uL
Prec 7-8 and Prec 7-10 endonuclease digestion test
H buffer 1uL plasmid 1uL EcoRI 0.15uL ddH2O 7.85uL
