IGEM:Peking/2007/Switch-Notebook/2007-7-19

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Experimenter: CXY, CCY

Contents

Colony PCR test of pCC002, Mach1-T1(1-9), JM110(1-9)

Colony PCR test of SS1-pMD18T-3, CD1-pMD18T-3

Plasmids are diluted to 1/50 of concentration before PCR.

  • Primers: Sul_S_1_F, Sul_S_1_R
  • Enzyme: Easy Taq

OK!! Incubate for Miniprep and digestion test tomorrow.

Colony PCR test of pLX007/8/9-lacZa

  • Primers: test_F_new, test_R
  • Enzyme: Easy Taq

pLX007-lacZa(1-21), pLX007 - Control, Marker, pLX008-lacZa(22-23)

pLX008-lacZa(24-41), Marker, pLX008-lacZa(42-47)

pLX008-lacZa(48-66), pLX008 – Control, Marker, pLX009-lacZa(67-70)

pLX009-lacZa(71-93), pLX009 – Control, Marker

pLX007-lacZa(8), pLX008-lacZa(40,41), pLX009-lacZa(68,88) OK!! Incubate for Miniprep and digestion test tomorrow.

prepare 48 tubes of 10mM Vb1, 1mL per tube

prepare 70% ethanol

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