IGEM:Peking/2007/Switch-Notebook/2007-7-17
From OpenWetWare
Jump to navigationJump to search
Experimenter: LCB, CXY
Contents
Electrophoresis for SD2
Re-do temperature PCR of SD2
Re-Re-do temperature PCR of CD2, third time(!!!) by NM
verify the concentration of LacZa(XhoI/SalI)
- got by Bobo on 9/7/07
too weak!.
digesting pLX007(1-6) with SalI or XhoI, respectively
double enzymes digest pLX007, pLX008 and pLX009 for getting vector
PCR for rec promoter
- Genome of GM1655 as temple