IGEM:Peking/2007/Switch-Notebook/2007-7-15

Experimenter: LXL, MHS

Contents

配1*TAE+EB

EB浓度加倍用于看pLX007

Single digestion test of pLX007

• Enzyme: PstI/ScaI/NdeI
• Plasmid: 2uL

l to r: pLX007-1(P/S/N), pLX007-2(P/S/N), pLX007-3(P/S/N), pLX007-4(P/S/N), Kit2(P/S/N), Marker, pLX007-4

Plasmid还看得很清, 但是酶切完全弥散了. pLX007作载体还是相当有问题啊.

今天晚上重新做阳转吧. 用测序的那管质粒好了.

CXY昨天PCR的东东

CD2大小差不多, 不过太稀了. 回收之后2uL看不到.

还是用temperature PCR试一下. 呼唤NM师兄!

Single digestion test of plasmid prepared yesterday (pLX007-lacZa)

• Enzyme: ScaI & PstI

l to r: pLX007-lacZa^(ScaI)(3,4,33,34), pLX007-lacZa^(Pst2)(1,2,31,32), Marker

可以切开的. ScaI的效率不高.

Double digestion test of plasmid prepared yesterday (pLX007-lacZa)

• Enzyme: PstI/XbaI

• Plasmid: 10uL
• PstI: 0.2uL
• XbaI: 0.2uL
• M buffer: 2uL
• H2O 8uL
• total: 20uL

l to r: pLX007-lacZa^^(PstI/XbaI)(5-25,27-29), Marker

l to r: Marker, pLX007-lacZa(30,35-53)

那些单切没切开的都是什么？