IGEM:Peking/2007/Switch-Notebook/2007-7-14

Experimenter: CDZ, MHS, LC, CXY

Contents

Transformation of SDA-H, Y, cro, lacZa-pCC002

• 8:15am, precipitate with ethanol: pCC002^^ (NotI/XhoI)
• 2:00pm, ligation of SDA-H, Y, cro, lacZa-pCC002 with pCC002^^ (4uL/1uL)
• 8:30pm, transformation

Sequencing result

CD1-2,4,5, SS1-2 are correct. Great! We have CD template.

RD1 is wrong. New primers already sent.

Miniprep and digestion test of pLX007-lacZa(1-53)

• H buffer: 2uL
• XhoI: 0.5uL
• NdeI: 0.5uL
• Plasmid: 10uL
• ddH2O: 7uL
• total: 20uL

l to r: pLX007-lacZa(1-23,25), marker

胶倒的不好。没连上应该看到300bp的带，连上了应该有500bp。都没有！质粒太少了？质粒不对？

Digestion test of pLX007(1)

l to r: pLX007(1)^^(NdeI/ScaI), marker

Not digested. The plasmid is wrong???