IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010/2010/08/04

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Léa
Miniprep of 10mL cultures elution in 35µL warm water

  • Ps/weak RBS/LuxR
  • Ps/med RBS/LuxR
  • Ps/stand RBS/LuxR
  • Pm/med RBS/LuxR
  • Pm/weak RBS/LuxR
  • Pm/stand RBS/LuxR
  • RBS/LuxI
  • Ps
  • Pm
  • Term


Nanodrop of minipreps

Sample name 260/280 260/230 ng/µL
Ps/weak RBS/LuxR 1.68 0.76 74.9
Ps/med RBS/LuxR 1.45 0.55 127.3
Ps/stand RBS/LuxR 1.73 0.93 62.8
Pm/med RBS/LuxR 1.68 0.91 134.5
Pm/weak RBS/LuxR 1.47 0.63 132.1
Pm/stand RBS/LuxR 1.85 1.60 118.9
RBS/LuxI 1.71 0.95 134.4
Ps 1.88 1.37 91.2
Pm 1.79 1.54 100.4
Term 1.88 1.65 85.6


Raphaël
Midiprep of 100mL culture

  • attC-t-attC-mRFP


Nanodrop of midiprep

Sample name 260/280 260/230 ng/µL
attC-t-attC-mRFP 1.89 2.27 370.2


Aleksandra and Raphaël
Overnight cultures in 10ml

  • attC/term/attC/mRFP #10, 11 cultures - Kan
  • term - Amp
  • RBS/LuxI/term in Turbo cells plate 1, 2 colonies - Amp
  • RBS/LuxI/term in Turbo cells plate 2, 2 colonies - Amp
  • RBS/LuxI/term in TOP10, 3 colonies - Amp