Raphaël
Glycerol 15%
2 glycerols per culture, one stored at -20°C and the other at -80°C
- TOP10 pSB1A2 weak RBS -TetA(C) - Amp
- TOP10 pSB1A2 med.RBS -TetA(C) - Amp
- TOP10 pSB1A2 weak RBS -LuxR - Amp
- TOP10 pSB1A2 med.RBS -LuxR - Amp
- TOP10 pSB1A2 stand.RBS -LuxR - Amp
Minipreps : 4 minipreps of a 10ml culture per biobrick, eluted with 30μL buffer and collected into 1 tube for a total volume of around 120μL:
- weak RBS -TetA(C) - Amp
- med.RBS -TetA(C) - Amp
- weak RBS -LuxR - Amp
- med.RBS -LuxR - Amp
- stand.RBS -LuxR - Amp
Nanodrop to determine the amount of DNA in the miniprep samples:
Sample name |
260/280 |
260/230 |
ng/μL
|
weak RBS -TetA(C) |
1.87 |
2.31 |
186.5
|
med.RBS -TetA(C) |
1.86 |
2.31 |
185.1
|
weak RBS -LuxR |
1.84 |
2.38 |
159.8
|
med.RBS -LuxR |
1.84 |
2.36 |
175.6
|
stand.RBS -LuxR |
1.85 |
2.35 |
191.7
|
|