IGEM:Paris Bettencourt 2012/Protocols/RCR Fusion

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  • Pipetting instructions:
5x Phusion HF Buffer
10 ul
10 mM dNTPs
1 uL
Forward primer
0.1 - 1.0 uM
Reverse primer
0.1 - 1.0 uM
Template DNA
10 pg - 1 ug
Water (Nuclease free)
to 50 ul
Total volume:
50 ul
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