IGEM:Paris Bettencourt 2012/Protocols/Plates

Plate color codes

Antibiotic type	Color code	Dilutant	Stock concentration	Dilution
Plain	black	-	-	-
Ampicilline	red	[math]\displaystyle{ H_{2}O }[/math] + filtration	100 mg/ml	x1000
Chloramphenicol	blue	ethanol 100%	30 mg/ml	x1000
Kanamycine	green	[math]\displaystyle{ H_{2}O }[/math] + filtration	100 mg/ml	x1000
Spectinomycine	orange	[math]\displaystyle{ H_{2}O }[/math] + filtration	25 mg/ml	x500/333

LB Agar (LBA) Protocol (500ml)

• Melt LBA in microwave (~8 min).
• Cool LBA before you can touch it without burn your skin (~4 min in water bath).
• Add required chemicals (antibiotic, etc).
• Add 24 ml of LBA into each plate.
• Wait while plates will be thicken.
• Dry them 45' under the flow hood, with the lid open.