IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output2009/06/16/Min Lin

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10:00 Gel (1-12M and 1-12O product)
Cut gel; Recycle the insert(about 1kb).
Wash with 30 ul Elution buffer, store in -20.

Gel to compare the concentration of insert (1-12M and 1-12O) and the vector(T7 promoter cut with SpeI and PstI)

Ligation 16 centigrade overnight

Ligase 1μL
Buffer 1μL
Vector 5μL
Insert 7μL

Transformation( ligation product into Top10)