IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/07/19/Zhangs
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Store the spawns of T7p+5J+CI+1-23L ①~⑤
Bacteria Liquid PCR to identify
System (Master mix is exhausted)
| 0.5μL | Tag enzyme |
| 1μL | 10× buffer |
| 2μL | dNTP |
| 0.25μL | Forward primer |
| 0.25μL | Reverse primer |
| 6μL | ddH2O |
| Template (Bacteria Liquid) | |
| 10μL | Total |
Electrophorsis
Result (from left to right)
Marker, T7p+5J+CI+1-23L ①~⑤, Control
The T7p+5J+CI+1-23L ②,④,⑤ are target plasmids.
