IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/06/22/Zhangs

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Double digestion for CI insert

Digestion system:

30μL CI plasmid
5μL 10×H beffer
1μL EcoRI
1μL SpeI
13μL ddH2O
50μL Total

Electrophoresis:
50μL system + 10μL buffer
5μL marker + 1μL buffer
Sample loading order: DL2000 marker, CI1, CI2

Extraction the nucleic acids from gel.