IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/06/15/Min Lin

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9:00
MiniPrep 1-12M and 1-12O.
Digest with EcoRI and XbaI

EcoRI 1μL
XbaI 1μL
10xM buffer 5μL
Plasmid 43μL

Transformation (pSalSer plasmid from Voigt Lab into Top10)

22:00
Digest 1-12M and 1-12O with EcoRI and SpeI overnight.

XbaI 1μL
PstI 1μL
10xM buffer 5μL
Plasmid 43μL

Digest T7 promoter with

SpeI 1μL
PstI 1μL
10xH buffer 5μL
Plasmid 43μL